| HDGF

Hepatoma-derived growth factor (HDGF) also known as high mobility group protein 1-like 2 (HMG-1L2) is a protein that is encoded by the HDGF gene.It is an acidic heparin-binding protein purified from the culture fluid of human hepatocellular carcinoma cell line HuH-7, which is the first member of this protein family to be discovered, and has the function of promoting cell proliferation, differentiation, tissue . It has the functions of promoting cell proliferation and differentiation, organ growth and development, and tissue repair after injury.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	400	200	100	50	25	12.5	6.25	0
OD	2.22	1.43	0.87	0.52	0.34	0.27	0.22	0.08
Corrected OD	2.14	1.35	0.79	0.44	0.26	0.19	0.14	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse HDGF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	86-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	104

 

| Linearity

Samples were spiked with high concentrations of Mouse HDGF and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108